Coding
PmeIR

Part:BBa_K093002:Design

Designed by: John Heil   Group: iGEM08_Waterloo   (2008-10-26)


PmeI endonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 652
    Illegal BsaI.rc site found at 345


Design Notes

The stop codon was changed to TAA.


Source

Synthetic from GENEART

References